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DSMZ

DSMZ 465h . MINERALMEDIUM WITH 6-METHYLNICOTINATE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Main sol. 465h #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 465 1000 ml - -
Selenite/tungstate stock solution 1 ml - -
6-methylnicotinate , adjust if required 5 g/l - -
Vitamin solution 1 ml - -
1 Supplement medium 465 with selenite, tungstate, 2.5 - 5 g/l 6-methylnicotinate from a stock solution (autoclave separately) and 1 ml/l vitamin solution (see medium 461).

Medium 465 #

1000
Solution occurs in 7 other media (first defined in Medium 465).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.50 g - -
KH2PO4 1.00 g - -
(NH4)2SO4 0.50 g - -
MgCl2 x 6 H2O 0.10 g - -
Ca(NO3)2 x 4 H2O 0.05 g - -
Trace element solution SL-4 1.00 ml - -
Distilled water 1000.00 ml - -
1 pH 7.25
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.

Selenite/tungstate stock solution #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 500 mg - -
Na2SeO3 x 5 H2O 3 mg - -
Na2WO4 x 2 H2O 4 mg - -
Distilled water 1000 ml - -
1 Autoclave separately. Add 1 ml per l medium.

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Medium 1 #

1000
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Medium 220 #

1000
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).

Medium 535 #

1000
Solution occurs in 18 other media (first defined in Medium 535).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) 		30 g - -
Agar 20 g - -
Distilled water 1000 ml - -
1 pH 7.3
2 Autoclave at 121°C for 15 min.

Medium 830 #

1000
Solution occurs in 9 other media (first defined in Medium 830).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g - -
Proteose peptone
(Difco no. 3) 		0.50 g - -
Casamino acids 0.50 g - -
Glucose 0.50 g - -
Starch
(soluble) 		0.50 g - -
Na-pyruvate 0.30 g - -
K2HPO4 0.30 g - -
MgSO4 x 7 H2O 0.05 g - -
Agar 15.00 g - -
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Metadata

Last modified: 19.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
6-methylnicotinate 5 -
Na2HPO4 x 2 H2O 3.5 24.655
KH2PO4 1 7.348
(NH4)2SO4 0.5 3.784
MgCl2 x 6 H2O 0.1 0.492
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
temperature mesophilic 2 strains