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DSMZ

DSMZ 465d . MINERAL MEDIUM WITH BENZYLCYANIDE AS NITROGEN SOURCE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Rhodococcus erythropolis DSM 9675

* Modification: Instead of 1.8 g/l glucose add 1.6 g/l sodium succinate from a sterile solution.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C

Main sol. 465d #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Main sol. 465 (modified) 1000.0 ml - -
Glucose* 1.8 g/l 1.8 9.991
Benzylcyanide 120.0 mg/l 0.12 -
Sodium succinate* 1.6 g/l - -
1 Prepare and autoclave medium 465 without ammonium sulfate. Add 120 mg/l benzylcyanide (not sterilized) to the incubation atmosphere or directly to the medium.

Main sol. 465 (modified) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.50 g 3.5 24.655
KH2PO4 1.00 g 1 7.348
MgCl2 x 6 H2O 0.10 g 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 g 0.05 0.212
Trace element solution SL-4 1.00 ml - -
Distilled water 1000.00 ml - -
1 pH 7.25
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 01.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 7
Strains with modifications: 2
Rhodococcus erythropolis DSM 9675

  1.6 g/l Sodium succinate
  Glucose

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 24.655
Sodium succinate 1.6 9.874
KH2PO4 1 7.348
Benzylcyanide 0.12 -
MgCl2 x 6 H2O 0.1 0.492
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Cultivation metadata from 7 strains BacDive

phyla 2 7 strains
temperature mesophilic 7 strains