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DSMZ

DSMZ 503b . FWM MEDIUM (PERCHLORATE)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Acidovorax sp. DSM 14692

* Modification: Omit solution E and acetate. Add 0.85 g/l NaNO3 to solution A and use 1.00 g/l pivalic acid as substrate.

Growth conditions: 28 °C

Main sol. 503b #

ml
Equipment needed: Filter Gassing station Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 950 ml - -
Solution B 30 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
Solution E* 10 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.0 - 7.2, if necessary.

Solution A #

950
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.211 1.547
NH4Cl 0.25 g 0.263 4.92
NaCl 1.00 g 1.053 18.012
MgCl2 x 6 H2O 0.40 g 0.421 2.071
KCl 0.50 g 0.526 7.06
CaCl2 x 2 H2O 0.15 g 0.158 1.074
Na2SO4 0.15 g 0.158 1.112
Modified Wolin's mineral solution 10.00 ml - -
NaNO3* 0.85 g - -
Distilled water 940.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-acetate* 1.36 g 136 1657.85
Pivalic acid* 1.00 g - -
Distilled water 10.00 ml - -

Solution D #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium perchlorate 1.22 g 122 996.406
Distilled water 10.00 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 167 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 09.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 4
Acidovorax sp. DSM 14692

-  Solution E
  0.85 g NaNO3
  1.00 g Pivalic acid
  Na-acetate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2CO3 1.499 14.138
Sodium perchlorate 1.219 9.954
0.999 -
NaNO3 0.849 9.991
KCl 0.5 6.7
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Cultivation metadata from 7 strains BacDive

phyla 1 7 strains
oxygen anaerobe 4 strains
oxygen aerobe 2 strains
temperature mesophilic 6 strains