DSMZ 816 . SPOROTOMACULUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 920 ml - -
Solution B 1 ml - -
Solution C 1 ml - -
Solution D 50 ml - -
Solution E 10 ml - -
Solution F 5 ml - -
Solution G 5 ml - -
Solution H 10 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, C, F, G, and H are autoclaved separately under 100% N2 gas. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere and autoclaved. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be checked and adjusted to 7.6 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary.
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.217 1.597
NH4Cl 0.30 g 0.326 6.096
KCl 0.50 g 0.543 7.29
NaCl 1.00 g 1.087 18.6
CaCl2 x 2 H2O 0.15 g 0.163 1.109
MgCl2 x 6 H2O 0.40 g 0.435 2.139
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Distilled water 920.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Selenite-tungstate solution 1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 2.5 g 50 471.752
Distilled water 50.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
3-Hydroxybenzoic acid 2.8 g 280 2027.22
Distilled water 10.0 ml - -
1 Neutralized with NaOH.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.5 g 100 -
Distilled water 5.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase
(BD BBL)
0.5 g 100 -
Distilled water 5.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
3-Hydroxybenzoic acid 2.8 20.272
Na2CO3 2.5 23.588
NaCl 1 17.112
KCl 0.5 6.707
Trypticase 0.5 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain