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DSMZ

DSMZ 816 . SPOROTOMACULUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 816 #

ml
Equipment needed: Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 920 ml - -
Solution B 1 ml - -
Solution C 1 ml - -
Solution D 50 ml - -
Solution E 10 ml - -
Solution F 5 ml - -
Solution G 5 ml - -
Solution H 10 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, C, F, G, and H are autoclaved separately under 100% N2 gas. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere and autoclaved. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be checked and adjusted to 7.6 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary.

Solution A #

920
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.217 1.597
NH4Cl 0.30 g 0.326 6.096
KCl 0.50 g 0.543 7.29
NaCl 1.00 g 1.087 18.6
CaCl2 x 2 H2O 0.15 g 0.163 1.109
MgCl2 x 6 H2O 0.40 g 0.435 2.139
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Distilled water 920.00 ml - -

Solution B #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Selenite-tungstate solution 1 ml - -

Solution D #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 2.5 g 50 471.752
Distilled water 50.0 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
3-Hydroxybenzoic acid 2.8 g 280 2027.22
Distilled water 10.0 ml - -
1 Neutralized with NaOH.

Solution F #

5
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.5 g 100 -
Distilled water 5.0 ml - -

Solution G #

5
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase
(BD BBL) 		0.5 g 100 -
Distilled water 5.0 ml - -

Solution H #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
3-Hydroxybenzoic acid 2.8 20.272
Na2CO3 2.5 23.588
NaCl 1 17.112
KCl 0.5 6.707
Trypticase 0.5 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain