830b
830b .
MEDIUM R2A FOR DENITRIFYING BACTERIA
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Aromatoleum toluvorans DSM 15124
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 830b #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | 0.5 | - | |
| Casamino acids | 0.50 | g | 0.5 | - | |
| Glucose | 0.50 | g | 0.5 | 2.775 | |
|
Starch
(soluble) |
0.50 | g | 0.5 | - | |
| Na-pyruvate | 0.30 | g | 0.3 | 2.726 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| KNO3 | 0.50 | g | 0.5 | 4.945 | |
| Trace element solution SL-10 | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare liquid medium under anaerobic conditions (N2). | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 20.12.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Aromatoleum toluvorans DSM 15124
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KNO3 | 0.5 | 4.945 |
| Glucose | 0.5 | 2.775 |
| Yeast extract | 0.5 | - |
| Proteose peptone | 0.5 | - |
| Casamino acids | 0.5 | - |
| Show more | ||
