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DSMZ

DSMZ 830d . R2A MEDIUM (MICROAEROBIC)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Thiomonas metallidurans DSM 25617

* Modification: Adjust pH of complete medium to 7.2.

Growth conditions: 7-14 days microaerobic 25 °C

Main sol. 830d #

ml
Equipment needed: Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g 0.5 -
Proteose peptone
(BD DIFCO no. 3) 		0.50 g 0.5 -
Casamino acids 0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Starch , soluble 0.50 g 0.5 -
Na-pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Distilled water 1000.00 ml - -
1 Dissolve ingredients, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes or serum vials) to 75% volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Adjust pH of the autoclaved medium to 5.0 - 5.5 using a sterile stock solution of sodium carbonate (5% w/v), prepared under 80% N2 and 20% CO2 gas mixture.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 7.2
Equipment needed: Autoclave, Gassing station
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Gas composition

  • 75 % N2
  • 15 % CO2
  • 10 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1
Thiomonas metallidurans DSM 25617

  7.2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 0.5 2.775
Starch 0.5 -
Casamino acids 0.5 -
Proteose peptone 0.5 -
Yeast extract 0.5 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen aerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 2 strains
temperature thermophilic 1 strain