Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
Trypticase soy broth
|1 Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution.|
|Medium type:||Complex medium|
|Equipment needed:||Autoclave, Gassing station, Hungate tubes|
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|Compound||Conc. [g/L]||Conc. [mM]|
|Trypticase soy broth||5.5||-|