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DSMZ

DSMZ 92a . MODIFIED MEDIUM 92

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 92a #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 92 990 ml - -
Vitamin solution 10 ml - -
1 Prepare medium 92 and subsequently add vitamins. Adjust pH to 7.0 - 7.2

Vitamin solution #

1000
Solution occurs in 3 other media (first defined in Medium 11a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Folic acid 2.00 mg 0.002 0.005
Pyridoxine hydrochloride 10.00 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.00 mg 0.005 0.013
Riboflavin 5.00 mg 0.005 0.013
Nicotinic acid 5.00 mg 0.005 0.041
Calcium pantothenate 5.00 mg 0.005 0.01
p-Aminobenzoic acid 1.00 mg 1.0e-3 0.007
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Distilled water 1000.00 ml - -

Medium 92 #

1000
Solution occurs in 1 other media (first defined in Medium 92).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth 30 g 30 -
Yeast extract 3 g 3 -
Agar , if necessary 15 g 15 44.599
Distilled water 1000 ml - -
1 Adjust pH to 7.0 - 7.2.

Metadata

Last modified: 11.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 29.7 -
Agar 14.85 44.153
Yeast extract 2.97 -
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen facultative anaerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain