DSMZ 964 . CAMINICELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA)
30.00 g 30 -
PIPES buffer
(SIGMA)
6.05 g 6.05 20.009
D-Glucose 5.00 g 5 27.753
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Sulfur
(powdered)
12.00 g 12 374.181
Peptone
(BD Bacto)
1.00 g 1 -
Yeast extract
(BD Bacto)
0.50 g 0.5 -
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except sulfur, peptone, yeast extract and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.2 - 7.4, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 110°C for 20 min. Add peptone, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 7.5.

Metadata

Last modified: 11.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 30 -
Sulfur 12 374.181
PIPES buffer 6.05 20.009
D-Glucose 5 27.753
Peptone 1 -
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 1 strain
temperature thermophilic 2 strains