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J1006. HALOPHILIC SOB MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.962 7.284
2 1.00 g 0.962 3.905
3 0.30 g 0.289 1.964
4 0.60 g 0.577 7.746
5 100.00 g 96.246 1646.86
6 1000.00 ml 960.738 53329
7
(1 M)
20.00 ml - -
8
(1 M)
3.00 ml - -
9
(1 M)
5.00 ml 0.007 0.138
10
(0.5 M)
1.00 ml 5.582 19.234
11
(*, 1 M)
10.00 ml 20.78 247.357
12
(see below, *)
0.06 ml - -
13 Trace vitamins
(*)
0.06 ml - -
14 Vitamin B12 solution
(*)
6.00 �l - -
Instructions:
  1. Mix components thoroughly and autoclave. Aseptically add the following solutions (autoclaved or *filter-sterilized): #7-14
  2. Adjust pH to 7.5.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(32%)
1.00 ml 0.405 11.118
2 2.00 g 1.998 -
3 0.05 g 0.05 0.808
4 0.05 g 0.05 0.366
5 0.03 g 0.03 -
6 0.05 g 0.05 -
7 0.05 g 0.05 0.206
8 0.05 g 0.05 0.375
9 0.20 g 0.2 -
10 0.05 g 0.05 -
11 0.05 g 0.05 -
12 0.05 g 0.05 -
13 1000.00 ml 997.21 55353.5
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 966.56 53652.2
NaCl 96.246 1646.86
NaHCO3 20.78 247.357
EDTA 5.582 19.234
(NH4)2SO4 0.962 7.284
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