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JCM 1139 . NATRONOSPIRA PROTEINIVORA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1139 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basic mineral salt medium 1 500 ml - -
Basal medium 2 500 ml - -
Tryptone
(autoclaved, 10%) 		15 ml - -
Yeast extract
(autoclaved) 		2 ml - -
1 Aseptically combine the above described media and solutions.

Basic mineral salt medium 1 #

2
Solution occurs in 1 other media (first defined in Medium J1139).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.0 g 120000 2053390
K2HPO4 2.5 g 1250 7176.65
(NH4)2SO4 0.5 g 250 1891.93
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 - 6.9 by 1 M K2HPO4 and autoclave. After cooling, add the following solutions (autoclaved):
MgCl2
(1 M) 		1.0 ml - -
Trace element solution 1.0 ml - -

Basal medium 2 #

7
Solution occurs in 1 other media (first defined in Medium J1139).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 190 g 27142.9 256094
NaHCO3 30 g 4285.71 51016.2
NaCl 16 g 2285.71 39112.2
K2HPO4 2 g 285.714 1640.38
1 Add components to distilled water and bring volume to 1.0 L. Check pH of the medium to be 9.9 - 10.0. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
NH4Cl
(2 M) 		4 ml - -
MgCl2
(1 M) 		1 ml - -
Trace element solution 2 ml - -

Casein solution 5%(w/v) #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein 5 g 50 24.248
Distilled water 100 ml - -
1 Suspend casein in distilled water and keep at 50C. Titrate pH to 8.2 - 8.5 with 1 M NaOH solution, until pH is stabilized. Autoclave the solution at 120C for 40 min in a closed bottle.

Trace element solution #

1000
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Metadata

Last modified: 24.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 60120.8 1028760
Na2CO3 13344.6 125907
NaHCO3 2107.04 25081.7
K2HPO4 755.022 4334.82
(NH4)2SO4 122.911 930.152
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Cultivation metadata from 2 strains BacDive

halophily extremely halophilic 1 strain
pH 8 - 10 2 strains
pH 10 - 12 2 strains
phyla 2 2 strains
nutrition organoheterotroph 2 strains
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