JCM 1227 . ZESTOSPHAERA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified Brock's salt base solution 1000.0000 ml - -
Na2S2O3 x 5 H2O 2.0000 g 0.995 4.009
NaOH 0.1600 g 0.08 1.99
Resazurin 1.0000 mg 5.0e-4 0.002
Trace mineral solution 10.0000 ml - -
Distilled water 1000.0000 ml - -
1 Heat the medium to boiling while bubbling with a N2-CO2 (4:1, v/v) gas mixture. After boiling, cool the medium in a water bath. Continue bubbling with the same gas mixture. While the medium is still warm, add the following:
Yeast extract
(Oxoid)
1.0000 g 0.498 -
Tryptone 1.0000 g 0.498 6.999
Casamino acids
(BD-Difco)
0.5000 g 0.249 -
L-Cysteine HCl x H2O 0.0625 g 0.031 -
2 After the medium is cooled to room temperature, adjust pH to 6.0 - 6.1. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave.
Solution occurs in 13 other media (first defined in Medium J165).
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 2.00 mg 0.002 0.007
MnCl2 x 4 H2O 1.80 mg 0.002 -
Na2B4O7 x 10 H2O 4.50 mg 0.005 -
ZnSO4 x 7 H2O 0.22 mg 2.2e-4 7.7e-4
CuCl2 x 2 H2O 0.05 mg 5.0e-5 2.9e-4
Na2MoO4 x 2 H2O 0.03 mg 3.0e-5 1.2e-4
VOSO4 x n H2O 0.03 mg 3.0e-5 -
CoSO4 x 7 H2O 0.01 mg 1.0e-5 6.5e-5
Distilled water 1000.00 ml - -
Solution occurs in 1 other media (first defined in Medium J446).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 -
CoCl2 x 6 H2O 0.15 g 0.15 1.155
MnCl2 x 4 H2O 0.10 g 0.1 -
FeSO4 x 7 H2O 0.10 g 0.1 0.36
ZnCl2 0.10 g 0.1 0.734
AlCl3 x 6 H2O 0.04 g 0.04 0.166
Na2WO2 x 2 H2O 0.03 g 0.03 -
CuCl2 x 2 H2O 0.02 g 0.02 0.117
NiSO4 x 6 H2O 0.02 g 0.02 0.129
H2SeO3 0.01 g 0.01 -
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 0.995 4.009
(NH4)2SO4 0.647 4.895
Tryptone 0.498 6.999
Yeast extract 0.498 -
Casamino acids 0.249 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature hyperthermophilic 1 strain