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J1294. MMJ/KT2 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1000.0 ml - -
2 1.0 mg 9.6e-4 0.004
3
(8%)
12.5 ml 2.07 24.641
4 Trace vitamins 10.0 ml - -
5 Vitamin B12 solution 0.2 ml - -
6
(5%)
10.0 ml - -
7
(5%)
10.0 ml - -
Instructions:
  1. Adjust pH to 7.0 and autoclave. After cooling, add the following solutions (filter-sterilized): #3-5
  2. Aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under N2): #6-7
  3. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25.00 g 24.753 423.536
2 4.20 g 4.158 20.455
3 3.40 g 3.366 13.658
4 0.50 g 0.495 6.64
5 0.25 g 0.248 4.627
6 0.14 g 0.139 0.796
7 0.70 g 0.693 4.714
8 0.02 g 0.02 0.071
9 Trace mineral solution 10.00 ml - -
10 1000.00 ml 988.324 54860.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace minerals 1000.000 ml - -
2 0.075 g 0.075 -
3 0.050 g 0.05 -
4 0.100 g 0.1 -
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 9.762 541.869
NaHCO3 2.07 24.641
Resazurin 9.6e-4 0.004
Pyridoxine x HCl 9.6e-5 -
Nicotinic acid 4.8e-5 3.9e-4
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