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J1315. METHANOGEN MS-I MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.450 g 0.437 7.476
2 0.200 g 0.194 2.311
3 0.090 g 0.087 0.355
4 0.225 g 0.218 1.605
5 0.225 g 0.218 1.254
6 0.225 g 0.218 1.653
7 0.060 g 0.058 0.396
8 2.000 mg 0.002 -
9 2.000 mg 0.002 0.007
10 Wolfe's mineral elixir 0.100 ml - -
11 1.000 mg 9.7e-4 0.004
12 1000.000 ml 969.133 53795
13 Trace vitamins
(*)
10.000 ml - -
14
(1.0 M)
10.000 ml 14.757 179.893
15
(5%)
10.000 ml - -
Instructions:
  1. Mix components thoroughly, except NaHCO3, and adjust pH to 7.0. Bring to a boil for a seconds, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or filter-sterilized, and stored under N2): #13-15
  2. Pressurize inoculated bottles to 100 KPa H2-CO2 (80:20, v/v).
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 8 other media (first defined in medium J470 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 30 121.714
2 5.00 g 5 -
3 10.00 g 10 171.108
4 1.00 g 1 3.597
5 1.80 g 1.8 -
6 1.00 g 1 6.802
7 1.80 g 1.8 -
8 0.10 g 0.1 -
9 0.18 g 0.18 -
10 0.10 g 0.1 1.617
11 0.10 g 0.1 0.413
12 2.80 g 2.8 -
13 0.10 g 0.1 -
14 0.10 g 0.1 0.529
15 1000.00 ml 998.207 55408.8
Instructions:
  1. First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 978.921 54338.3
Sodium acetate 14.757 179.893
NaCl 0.438 7.492
(NH4)2SO4 0.218 1.653
KH2PO4 0.218 1.605
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