JCM 1315 . METHANOGEN MS-I MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 0.450 g 0.437 7.476
NaHCO3 0.200 g 0.194 2.311
MgSO4 x 7 H2O 0.090 g 0.087 0.355
KH2PO4 0.225 g 0.218 1.605
K2HPO4 0.225 g 0.218 1.254
(NH4)2SO4 0.225 g 0.218 1.653
CaCl2 x 2 H2O 0.060 g 0.058 0.396
(NH4)2Ni(SO4)2 x 6 H2O 2.000 mg 0.002 0.005
FeSO4 x 7 H2O 2.000 mg 0.002 0.007
Wolfe's mineral elixir 0.100 ml - -
Resazurin 1.000 mg 9.7e-4 0.004
Distilled water 1000.000 ml - -
1 Mix components thoroughly, except NaHCO3, and adjust pH to 7.0. Bring to a boil for a seconds, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or filter-sterilized, and stored under N2):
Trace vitamins
(*)
10.000 ml - -
Sodium acetate
(1.0 M)
10.000 ml - -
Na2S x 9 H2O
(5%)
10.000 ml - -
2 Pressurize inoculated bottles to 100 KPa H2-CO2 (80:20, v/v).
Solution occurs in 8 other media (first defined in Medium J470).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x n H2O 5.00 g 5 -
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 -
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.438 7.493
(NH4)2SO4 0.218 1.653
KH2PO4 0.218 1.605
K2HPO4 0.218 1.254
NaHCO3 0.194 2.311
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