J1315
1315 .
METHANOGEN MS-I MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1315 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 0.450 | g | 0.437 | 7.476 | |
| NaHCO3 | 0.200 | g | 0.194 | 2.311 | |
| MgSO4 x 7 H2O | 0.090 | g | 0.087 | 0.355 | |
| KH2PO4 | 0.225 | g | 0.218 | 1.605 | |
| K2HPO4 | 0.225 | g | 0.218 | 1.254 | |
| (NH4)2SO4 | 0.225 | g | 0.218 | 1.653 | |
| CaCl2 x 2 H2O | 0.060 | g | 0.058 | 0.396 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.000 | mg | 0.002 | 0.005 | |
| FeSO4 x 7 H2O | 2.000 | mg | 0.002 | 0.007 | |
| Wolfe's mineral elixir | 0.100 | ml | - | - | |
| Resazurin | 1.000 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly, except NaHCO3, and adjust pH to 7.0. Bring to a boil for a seconds, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or filter-sterilized, and stored under N2): | |||||
|
Trace vitamins
(*) |
10.000 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
10.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.000 | ml | - | - | |
| 2 Pressurize inoculated bottles to 100 KPa H2-CO2 (80:20, v/v). | |||||
Solution occurs in 8
other media (first defined in Medium J470).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
| MnSO4 x n H2O | 5.00 | g | 5 | - | |
| NaCl | 10.00 | g | 10 | 171.116 | |
| FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
| CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
| CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
| ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
| CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
| AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | - | |
| H3BO3 | 0.10 | g | 0.1 | 1.617 | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
| Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
| Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |