J1319
1319 .
MODIFIED ACETOBACTER MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1319 #
ml
Equipment needed: Hungate tubes
Anaerobic workbench
Filter
Autoclave
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.98 | 18.329 | |
| KH2PO4 | 0.33 | g | 0.324 | 2.377 | |
| K2HPO4 | 0.45 | g | 0.441 | 2.533 | |
| NaCl | 10.00 | g | 9.804 | 167.76 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.098 | 0.398 | |
| Trace minerals | 10.00 | ml | - | - | |
| Yeast extract | 2.00 | g | 1.961 | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 910.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
50.00 | ml | - | - | |
|
Fructose
(1.0 M) |
20.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
| 2 Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N2): | |||||
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |