Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.5 | g | 0.494 | 2.837 | |
KH2PO4 | 0.5 | g | 0.494 | 3.631 | |
NH4Cl | 1.0 | g | 0.988 | - | |
Na2SO4 | 1.0 | g | 0.988 | 6.957 | |
MgSO4 x 7 H2O | 2.0 | g | 1.976 | 8.018 | |
CaCl2 x 2 H2O | 0.1 | g | 0.099 | 0.672 | |
Sodium lactate | 2.0 | g | 1.976 | 17.636 | |
Yeast extract | 1.0 | g | 0.988 | - | |
FeCl2 solution | 1.0 | ml | - | - | |
Trace element solution | 1.0 | ml | - | - | |
Trace vitamins | 10.0 | ml | - | - | |
Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
Distilled water | 980.0 | ml | - | - | |
1 SolutionB: | |||||
FeSO4 x 7 H2O | 0.1 | g | 0.099 | 0.355 | |
Distilled water | 10.0 | ml | - | - | |
2 SolutionC: | |||||
Sodium thioglycolate | 0.1 | g | 0.099 | 0.866 | |
Ascorbic acid | 0.1 | g | 0.099 | 0.561 | |
Distilled water | 10.0 | ml | - | - | |
3 Solution A: | |||||
4 Dissolve ingredients of each solution in the appropriate amount of distilled water. Bring solution A to a boil and cool down to room temperature under a N2 gas stream. Add solutions Band C to the medium, then, adjust pH to 7.3 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 7.3 |
Equipment needed: | Autoclave |
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