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JCM 366 . VULCANITHERMUS MEDIATLANTICUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J366 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.324 6.048
KCl 0.33 g 0.324 4.34
KH2PO4 0.33 g 0.324 2.377
CaCl2 x 2 H2O 0.33 g 0.324 2.201
MgCl2 x 6 H2O 0.33 g 0.324 1.591
NaCl 30.00 g 29.412 503.281
Yeast extract
(BD-Difco) 		0.30 g 0.294 -
Sodium pyruvate 2.00 g 1.961 17.819
NaNO3 1.00 g 0.98 11.535
Trace minerals 10.00 ml - -
Trace vitamins 10.00 ml - -
Distilled water 1000.00 ml - -
1 Mix ingredients except CaCl2 x 2H2O and MgCl2 x 6H2O, adjust pH to 6.5, and autoclave. Separately autoclave CaCl2 x 2H2O and MgCl2 x 6H2O, and add to the medium. Dispense the medium into culture vessels under a N2 atmosphere, and seal with butyl rubber stoppers.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.422 503.449
Sodium pyruvate 1.961 17.819
NaNO3 0.98 11.535
CaCl2 x 2 H2O 0.325 2.207
NH4Cl 0.324 6.048
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature thermophilic 1 strain