J451
451 .
ANOXIC MEDIUM FOR STRAIN AcBE2-1
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J451 #
ml
| 1 Use sodium acetate instead of 17 beta-estradiol donor. The former can be added prior to autoclaving. After autoclaving and cooling under N2-CO2 (80:20, v/v), add the following components to the medium: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.000 | g | 0.969 | 16.581 | |
| MgCl2 x 6 H2O | 0.400 | g | 0.388 | 1.907 | |
| KH2PO4 | 0.200 | g | 0.194 | 1.424 | |
| KCl | 0.500 | g | 0.484 | 6.499 | |
| NH4Cl | 0.250 | g | 0.242 | 4.529 | |
| CaCl2 x 2 H2O | 0.150 | g | 0.145 | 0.989 | |
| Na2SO4 | 0.070 | g | 0.068 | 0.478 | |
| NaNO3 | 0.425 | g | 0.412 | 4.845 | |
| Distilled water | 1000.000 | ml | - | - | |
|
84 g/L NaHCO3 solution
(autoclaved under CO2 atmosphere) |
30.000 | ml | - | - | |
| Trace element solution SL-10 | 1.000 | ml | - | - | |
| Selenite-tungstate solution | 1.000 | ml | - | - | |
| 7 Vitamins solution | 0.500 | ml | - | - | |
| 2 The final pH of the medium should be about 7.2. All culture vessels with estradiol as substrate are incubated at 28C in the dark for 8 - 10 days. The culture with estradiol should be briefly shaken once a day. | |||||
Solution occurs in 20
other media (first defined in Medium J433).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. | |||||
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium J558).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 4 | mg | 0.04 | 0.03 |
| D-Biotin | 2 | mg | 0.02 | 0.082 |
| D-Calcium pantothenate | 5 | mg | 0.05 | - |
| Thiamine HCl | 10 | mg | 0.1 | - |
| p-Aminobenzoic acid | 10 | mg | 0.1 | 0.729 |
| Nicotinic acid | 20 | mg | 0.2 | 1.625 |
| Pyridoxine hydrochloride | 50 | mg | 0.5 | - |
| Distilled water | 100 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.2 |
| Edit in Medium builder | |