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JCM 478 . MODIFIED MMJHS MEDIUM FOR GO25

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J478 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.782 338.508
K2HPO4 0.90 g 0.89 5.111
KH2PO4 0.70 g 0.692 5.088
CaCl2 x 2 H2O 0.80 g 0.791 5.382
NH4Cl 0.25 g 0.247 4.623
NaNO3 1.00 g 0.989 11.637
MgSO4 x 7 H2O 4.00 g 3.956 16.052
MgCl2 x 6 H2O 3.00 g 2.967 14.596
KCl 0.33 g 0.326 4.378
NiCl2 x 6 H2O 1.00 mg 9.9e-4 0.008
Na2SeO3 x 5 H2O 1.00 mg 9.9e-4 0.004
Fe2(SO4)3 x n H2O 0.01 g 0.01 -
H2WO4 1.00 mg 9.9e-4 0.004
Na2S2O3 x 5 H2O 2.00 g 1.978 7.971
Trace mineral solution 10.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 2.00 g 1.978 23.549
Sulfur
(powder) 		10.00 g 9.891 308.425
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients except Trace vitamins, NaHCO3 and sulfur, adjust pH to 6.8 and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.2 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J368).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.02 g 0.02 0.077
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
SrCl2 x 6 H2O 0.01 g 0.01 0.038
NaBr 0.01 g 0.01 0.097
KI 0.01 g 0.01 0.039
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.782 338.508
Sulfur 9.891 308.425
MgSO4 x 7 H2O 3.956 16.052
MgCl2 x 6 H2O 2.967 14.596
NaHCO3 1.978 23.549
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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