Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2HPO4 x 2 H2O | 0.530 | g | 0.587 | 4.135 |
KH2PO4 | 0.410 | g | 0.454 | 3.336 |
NaNO3 | 0.850 | g | 0.941 | 11.075 |
Sodium acetate x 3 H2O | 0.136 | g | 0.151 | - |
FeCl2 solution | 1.000 | ml | - | - |
Trace element solution | 1.000 | ml | - | - |
Selenite-tungstate solution | 1.000 | ml | - | - |
Distilled water | 900.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.11 | g | 2.2 | - |
MgCl2 x 6 H2O | 0.10 | g | 2 | 9.838 |
Distilled water | 50.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4HCO3 | 0.44 | g | 6.984 | - | |
NaHCO3 | 3.73 | g | 59.206 | 704.779 | |
Na2SO4 | 0.20 | g | 3.175 | 22.35 | |
Distilled water | 50.00 | ml | - | - | |
1 Prepare all solutions and autoclave under a N2 atmosphere. After cooling, combine the solutions and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Then, aseptically and anaerobically add the following autoclaved or *filter-sterilized solutions to the medium: | |||||
Sodium acetate
(1.0 M) |
10.00 | ml | - | - | |
Vitamin solution
(*) |
1.00 | ml | - | - | |
Thiamine solution
(*) |
1.00 | ml | - | - | |
Vitamin B12 solution 0.05 g/L
(*) |
1.00 | ml | - | - | |
2 Check the final pH of the medium to be around 7.3. | |||||
3 Comment: JCM 14587 grows also in a modified medium with benzene and chlorate. For preparation of the modified medium, replace NaNO3 with 1.06 g NaClO3(in 900 ml solution A). After combining all solutions (A, B and C), add 1.0 ml/L of 1.0 M sodium acetate solution (autoclaved), 25 ml/L of 20 mM benzene (water-saturated, autoclaved), and 1 ml/L of each of vitamin solution (see Medium No. 403, filter-sterilized), thiamine solution (see Medium No. 403, filter-sterilized) and vitamin B12 solution (see Medium No. 403, filter-sterilized) to the medium. Otherwise, prepare the modified medium as described above. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
p-Aminobenzoic acid | 4 | mg | 0.04 | 0.292 |
Biotin | 1 | mg | 0.01 | 0.041 |
Nicotinic acid | 10 | mg | 0.1 | 0.812 |
DL-Calcium pantothenate | 5 | mg | 0.05 | 0.105 |
Pyridoxine hydrochloride | 10 | mg | 0.1 | - |
Sodium phosphate buffer
(10 mM, pH 7.1) |
100 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Thiamine HCl | 10 | mg | 0.1 | - |
Sodium phosphate buffer
(25 mM, pH 3.4) |
100 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 5 | mg | 0.05 | 0.037 |
Distilled water | 100 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.3 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |