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JCM 594 . ALICYCLIPHILUS DENITRIFICANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

903
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.530 g 0.587 4.135
KH2PO4 0.410 g 0.454 3.336
NaNO3 0.850 g 0.941 11.075
Sodium acetate x 3 H2O 0.136 g 0.151 -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Selenite-tungstate solution 1.000 ml - -
Distilled water 900.000 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.11 g 2.2 -
MgCl2 x 6 H2O 0.10 g 2 9.838
Distilled water 50.00 ml - -

Solution C #

63
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4HCO3 0.44 g 6.984 -
NaHCO3 3.73 g 59.206 704.779
Na2SO4 0.20 g 3.175 22.35
Distilled water 50.00 ml - -
1 Prepare all solutions and autoclave under a N2 atmosphere. After cooling, combine the solutions and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Then, aseptically and anaerobically add the following autoclaved or *filter-sterilized solutions to the medium:
Sodium acetate
(1.0 M) 		10.00 ml - -
Vitamin solution
(*) 		1.00 ml - -
Thiamine solution
(*) 		1.00 ml - -
Vitamin B12 solution 0.05 g/L
(*) 		1.00 ml - -
2 Check the final pH of the medium to be around 7.3.
3 Comment: JCM 14587 grows also in a modified medium with benzene and chlorate. For preparation of the modified medium, replace NaNO3 with 1.06 g NaClO3(in 900 ml solution A). After combining all solutions (A, B and C), add 1.0 ml/L of 1.0 M sodium acetate solution (autoclaved), 25 ml/L of 20 mM benzene (water-saturated, autoclaved), and 1 ml/L of each of vitamin solution (see Medium No. 403, filter-sterilized), thiamine solution (see Medium No. 403, filter-sterilized) and vitamin B12 solution (see Medium No. 403, filter-sterilized) to the medium. Otherwise, prepare the modified medium as described above.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Thiamine solution #

100
Solution occurs in 10 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.1 -
Sodium phosphate buffer
(25 mM, pH 3.4) 		100 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain