J684
684 .
ANAEROBIC TYEG MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J684 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.75 | g | 0.73 | 5.366 | |
| K2HPO4 | 1.50 | g | 1.461 | 8.386 | |
| NaCl | 0.90 | g | 0.876 | 14.996 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.195 | 0.958 | |
| NH4Cl | 0.90 | g | 0.876 | 16.383 | |
| Trace mineral solution | 9.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
|
FeSO4 x 7 H2O
(10%) |
5.00 | �l | - | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 1.947 | 27.398 | |
| Yeast extract | 2.00 | g | 1.947 | - | |
| Glucose | 2.00 | g | 1.947 | 10.809 | |
| Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Solution occurs in 4
other media (first defined in Medium J684).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.50 | g | 12.5 | 65.397 | |
| FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | - | |
| CoCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 0.20 | g | 0.2 | 1.488 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 0.02 | g | 0.02 | 0.076 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 1.947 | 27.398 |
| Glucose | 1.947 | 10.809 |
| Yeast extract | 1.947 | - |
| K2HPO4 | 1.461 | 8.386 |
| NaCl | 0.885 | 15.146 |
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