JCM 684 . ANAEROBIC TYEG MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.75 g 0.73 5.366
K2HPO4 1.50 g 1.461 8.386
NaCl 0.90 g 0.876 14.996
MgCl2 x 6 H2O 0.20 g 0.195 0.958
NH4Cl 0.90 g 0.876 16.383
Trace mineral solution 9.00 ml - -
Trace vitamins 10.00 ml - -
FeSO4 x 7 H2O
(10%)
5.00 �l - -
Tryptone
(BD-Difco)
2.00 g 1.947 27.398
Yeast extract 2.00 g 1.947 -
Glucose 2.00 g 1.947 10.809
Resazurin 1.00 mg 9.7e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter):
Na2S x 9 H2O
(5%)
8.00 ml - -
Solution occurs in 4 other media (first defined in Medium J684).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.50 g 12.5 65.397
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 -
CoCl2 x 6 H2O 0.02 g 0.02 0.154
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.20 g 0.2 1.488
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.02 g 0.02 0.076
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 1.947 27.398
Glucose 1.947 10.809
Yeast extract 1.947 -
K2HPO4 1.461 8.386
NaCl 0.885 15.146
Show more

Cultivation metadata from 3 strains BacDive

pH 6 - 8 3 strains
pH 8 - 10 3 strains
pH 4 - 6 2 strains
phyla 1 3 strains
oxygen anaerobe 3 strains
Show more