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JCM 808 . SULFURIMONAS GD1 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J808 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.500 g 5.324 91.107
MgCl2 x 6 H2O 2.280 g 2.207 10.857
CaCl2 x 2 H2O 0.340 g 0.329 2.239
KCl 0.150 g 0.145 1.948
Na2SO4 0.910 g 0.881 6.202
NaHCO3 0.200 g 0.194 2.305
KBr 0.023 g 0.022 0.187
H3BO3 5.700 mg 0.006 0.089
SrCl2 x 6 H2O 9.100 mg 0.009 0.033
NH4Cl 4.900 mg 0.005 0.089
KH2PO4 1.200 mg 0.001 0.009
NaF 0.670 mg 6.5e-4 0.015
HEPES 2.380 g 2.304 9.668
Distilled water 1000.000 ml - -
1 Mix ingredients and adjust pH to 7.3. Fill the medium in a glass bottle, bubble with N2 for 30 min, then, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Trace vitamins 10.000 ml - -
Selenite-tungstate solution 1.000 ml - -
KNO3
(10%) 		10.000 ml - -
Na2S2O3 x 5 H2O
(10%) 		10.000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 5.324 91.107
HEPES 2.304 9.668
MgCl2 x 6 H2O 2.207 10.857
Na2SO4 0.881 6.202
CaCl2 x 2 H2O 0.329 2.239
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
temperature psychrophilic 1 strain