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J981. ANAEROBIC BICARBONATE-BUFFERED MEDIUM WITH PHENOL

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.189 1.392
2 0.45 g 0.426 7.966
3 1.70 g 1.61 27.546
4 0.50 g 0.473 6.351
5 0.40 g 0.379 1.863
6 0.16 g 0.152 1.031
7 4.00 g 3.788 26.667
8 FeCl2 solution 1.00 ml - -
9 Trace element solution 1.00 ml - -
10 Selenite-tungstate solution 1.00 ml - -
11 1.00 mg 9.5e-4 0.004
12 1000.00 ml 945.272 52470.5
13
(8.0%)
30.00 ml 4.907 58.41
14 Vitamin solution 1.00 ml - -
15 Thiamine solution 1.00 ml - -
16 Vitamin B12 solution 1.00 ml - -
17
(freshly prepared, *, 0.1 M)
10.00 ml 10.038 106.66
18
(5%)
10.00 ml - -
Instructions:
  1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized): #13-16
  2. Distribute the medium into sterile culture vessels (e.g., 50 ml per 120 ml bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with sterile butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere) in the order listed: #17-18
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 949.044 52679.9
Phenol 10.038 106.66
NaHCO3 4.907 58.41
Na2SO4 3.788 26.667
NaCl 1.61 27.546
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