Back to all solutions

Main sol. 514

Internal Solution-ID: S1134

This solution is used in 5 mediaand was first defined in medium 514.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone 5.00 g 5 -
Yeast extract
(Bacto )
1.00 g 1 -
Fe(III) citrate 0.10 g 0.1 0.408
NaCl 19.45 g 19.45 332.82
MgCl2
(anhydrous)
5.90 g 5.9 61.968
Na2SO4 3.24 g 3.24 22.811
CaCl2 1.80 g 1.8 16.219
KCl 0.55 g 0.55 7.378
NaHCO3 0.16 g 0.16 1.905
KBr 0.08 g 0.08 0.672
SrCl2 34.00 mg 0.034 0.214
H3BO3 22.00 mg 0.022 0.356
Na-silicate 4.00 mg 0.004 0.033
NaF 2.40 mg 0.002 0.057
(NH4)NO3 1.60 mg 0.002 0.02
Na2HPO4 8.00 mg 0.008 0.056
Distilled water 1000.00 ml - -
1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.
514a HALF STRENGTH BACTO MARINE BROTH
514 BACTO MARINE BROTH (DIFCO 2216)
514b MEDIUM 514 plus additional salt
760 MARINE THERMOCOCCUS MEDIUM
1013 HALOBACTERIOVORAX MEDIUM