Name: HALF STRENGTH BACTO MARINE BROTH
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 514a #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Medium 514	500	ml	-	-
Distilled water	500	ml	-	-
Agar , for solid medium	15	g/l	15	44.599
1 1/2 strength medium 514. If using Difco Bacto Marine Broth use 18.7 g/l.
2 Final pH 7.6. The medium may be solidified by adding 15 g/l agar.

Medium 514 #

1000

Solution occurs in 4 other media (first defined in Medium 514).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Bacto peptone	5.00	g	5	-
Yeast extract (Bacto )	1.00	g	1	-
Fe(III) citrate	0.10	g	0.1	0.408
NaCl	19.45	g	19.45	332.82
MgCl₂ (anhydrous)	5.90	g	5.9	61.968
Na₂SO₄	3.24	g	3.24	22.811
CaCl₂	1.80	g	1.8	16.219
KCl	0.55	g	0.55	7.378
NaHCO₃	0.16	g	0.16	1.905
KBr	0.08	g	0.08	0.672
SrCl2	34.00	mg	0.034	0.214
H₃BO₃	22.00	mg	0.022	0.356
Na-silicate	4.00	mg	0.004	0.033
NaF	2.40	mg	0.002	0.057
(NH₄)NO₃	1.60	mg	0.002	0.02
Na₂HPO₄	8.00	mg	0.008	0.056
Distilled water	1000.00	ml	-	-
1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.
2 Note: M.514 refers to Bacto Marine Broth Difco 2216 (BD 279110). Other brands (even if they refer to Difco2216) have not been tested and might not support growth for some strains.

All strains for this medium:	39
Strains with modifications:	5

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123	MARINE AGAR
514	BACTO MARINE BROTH (DIFCO 2216)
a	HALF STRENGTH BACTO MARINE BROTH
b	MEDIUM 514 plus additional salt
c	REACTIVATION WITH LIQUID MEDIUM 514
d	MODIFIED BACTO MARINE BROTH
e	MARINE BROTH (ANAEROB)
f	MARINE BROTH (ROTH CP73)
g	HALF STRENGTH BACTO MARINE BROTH with 50% Seawater
760	MARINE THERMOCOCCUS MEDIUM
1173	MODIFIED MEDIUM 514