DSMZ 514b . MEDIUM 514 plus additional salt

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 514 1000.0 ml - -
Agar , for solid medium
(adjust in required in range 15-20)
17.5 g/l 17.5 52.032
1 Use medium 514 to which is added NaCl at the concentration indicated (i.e. "514 + 6% NaCl" indicates use medium 514 to which 60 g/l NaCl has been added; see strain dependant modifications).
2 Final pH 7.6. The medium may be solidified by adding 15 - 20 g/l agar.
Solution occurs in 4 other media (first defined in Medium 514).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone 5.00 g 5 -
Yeast extract
(Bacto )
1.00 g 1 -
Fe(III) citrate 0.10 g 0.1 0.408
NaCl 19.45 g 19.45 332.82
MgCl2
(anhydrous)
5.90 g 5.9 61.968
Na2SO4 3.24 g 3.24 22.811
CaCl2 1.80 g 1.8 16.219
KCl 0.55 g 0.55 7.378
NaHCO3 0.16 g 0.16 1.905
KBr 0.08 g 0.08 0.672
SrCl2 34.00 mg 0.034 0.214
H3BO3 22.00 mg 0.022 0.356
Na-silicate 4.00 mg 0.004 0.033
NaF 2.40 mg 0.002 0.057
(NH4)NO3 1.60 mg 0.002 0.02
Na2HPO4 8.00 mg 0.008 0.056
Distilled water 1000.00 ml - -
1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.

Metadata

Last modified: 19.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6
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Associated strains

All strains for this medium: 51
Strains with modifications: 30

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
Agar 17.5 52.032
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
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Cultivation metadata from 51 strains BacDive

halophily moderately halophilic 2 strains
pH 6 - 8 15 strains
pH 8 - 10 12 strains
pH 4 - 6 11 strains
pH 10 - 12 1 strain
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