1173
1173 .
MODIFIED MEDIUM 514
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1173 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto peptone | 10.00 | g | 10 | - | |
|
Yeast extract
(Bacto ) |
5.00 | g | 5 | - | |
| Fe(III) citrate | 0.10 | g | 0.1 | 0.408 | |
| NaCl | 39.45 | g | 39.45 | 675.051 | |
|
MgCl2
(anhydrous) |
5.90 | g | 5.9 | 61.968 | |
| Na2SO4 | 3.24 | g | 3.24 | 22.811 | |
| CaCl2 | 1.80 | g | 1.8 | 16.219 | |
| KCl | 0.55 | g | 0.55 | 7.378 | |
| NaHCO3 | 0.16 | g | 0.16 | 1.905 | |
| KBr | 0.08 | g | 0.08 | 0.672 | |
| SrCl2 | 34.00 | mg | 0.034 | 0.214 | |
| H3BO3 | 22.00 | mg | 0.022 | 0.356 | |
| Na-silicate | 4.00 | mg | 0.004 | 0.033 | |
| NaF | 2.40 | mg | 0.002 | 0.057 | |
| (NH4)NO3 | 1.60 | mg | 0.002 | 0.02 | |
| Na2HPO4 | 8.00 | mg | 0.008 | 0.056 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH should be 7.6 ± 0.2 at 25°C. | |||||
Metadata
| Last modified: | 26.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.4 - 7.8 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 39.45 | 675.051 |
| Bacto peptone | 10 | - |
| MgCl2 | 5.9 | 61.968 |
| Yeast extract | 5 | - |
| Na2SO4 | 3.24 | 22.811 |
| Show more | ||
