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514. BACTO MARINE BROTH (DIFCO 2216)

Culture medium recipe

Vol.
×

M 514 #

ml
Solution occurs in 2 other media (first defined in medium 514 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 -
2 1.00 g 1 -
3 0.10 g 0.1 0.408
4 19.45 g 19.45 332.806
5
(anhydrous)
5.90 g 5.9 61.968
6 3.24 g 3.24 22.81
7 1.80 g 1.8 16.219
8 0.55 g 0.55 7.378
9 0.16 g 0.16 1.905
10 0.08 g 0.08 0.672
11 34.00 mg 0.034 0.214
12 22.00 mg 0.022 0.356
13 4.00 mg 0.004 0.033
14 2.40 mg 0.002 0.057
15 1.60 mg 0.002 0.02
16 8.00 mg 0.008 0.056
17 1000.00 ml 998.207 55408.8
Instructions:
  1. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Taxonomic range:  Phages
Medium type: Complex medium
Final pH: 7.6

Associated strains

All strains for this medium: 1914
Strains with modifications: 244
514 BACTO MARINE BROTH (DIFCO 2216)
a HALF STRENGTH BACTO MARINE BROTH
b MEDIUM 514 PLUS ADDITIONAL SALT
c REACTIVATION WITH LIQUID MEDIUM 514
d MODIFIED BACTO MARINE BROTH
e MARINE BROTH (ANAEROB)
f MARINE BROTH (ROTH CP73)

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 19.45 332.806
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.81
CaCl2 1.8 16.219
Bacto yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III)citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
NH4NO3 0.002 0.02

Cultivation metadata from 1914 strains BacDive

halophily moderate 572 strains
halophily not 512 strains
halophily slightly 364 strains
halophily extreme 91 strains
pH 6 - 8 604 strains
pH 8 - 10 547 strains
pH 4 - 6 448 strains
pH 10 - 14 207 strains
pH 2 - 4 10 strains
phyla 13 1815 strains
nutrition chemoorganotroph 15 strains
nutrition chemoheterotroph 11 strains
nutrition heterotroph 8 strains
nutrition chemoorganoheterotroph 3 strains
nutrition autotroph 1 strain
oxygen aerobe 549 strains
oxygen facultative anaerobe 94 strains
oxygen obligate aerobe 48 strains
oxygen anaerobe 26 strains
oxygen facultative aerobe 17 strains
oxygen microaerophile 7 strains
oxygen obligate anaerobe 2 strains
temperature mesophilic 1582 strains
temperature psychrophilic 319 strains
temperature thermophilic 79 strains
temperature hyperthermophilic 3 strains

Similar media

1517. MODIFIED BACTO MARINE BROTH (DIFCO 2216) 97 %
1173. MODIFIED MEDIUM 514 97 %
514b. MEDIUM 514 PLUS ADDITIONAL SALT 87 %
1354. BACTO MARINE BROTH WITH NA-ACETATE 87 %
514a. HALF STRENGTH BACTO MARINE BROTH 82 %

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