Trace element solution (Vishniac & Santer, 1957)
Internal Solution-ID: S114
This solution is used in 5 media and was first defined in medium 69.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 50.00 | g | 50 | 148.718 | |
| ZnSO4 x 7 H2O | 22.00 | g | 22 | 76.506 | |
| CaCl2 x 2 H2O | 5.54 | g | 5.54 | 37.683 | |
| MnCl2 x 4 H2O | 5.06 | g | 5.06 | 25.568 | |
| FeSO4 x 7 H2O | 5.00 | g | 5 | 17.985 | |
| (NH4)6Mo7O24 x 4 H2O | 1.10 | g | 1.1 | 0.89 | |
| CuSO4 x 5 H2O | 1.57 | g | 1.57 | 6.288 | |
| CoCl2 x 6 H2O | 1.61 | g | 1.61 | 12.4 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH. | |||||