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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: CaSO4

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86 CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 1 14.069
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
Nitrilotriacetic acid 0.1 0.523
MgSO4 x 7 H2O 0.1 0.406
CaSO4 x 2 H2O 0.06 0.348
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86a MODIFIED CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 25 74.332
Peptone 3 -
Monosodium glutamate 1 5.913
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
Nitrilotriacetic acid 0.1 0.523
MgSO4 x 7 H2O 0.1 0.406
CaSO4 x 2 H2O 0.06 0.348
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155 BEGGIATOA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 10 29.733
Na-acetate 0.5 6.095
Nutrient broth 0.5 -
FeSO4 x 7 H2O 0.004 0.013
EDTA 1.0e-3 0.003
NH4Cl 4.5e-4 0.008
MgSO4 x 7 H2O 2.0e-4 8.1e-4
K2HPO4 1.0e-4 5.7e-4
H3BO3 5.0e-5 8.1e-4
ZnSO4 x 7 H2O 5.0e-5 1.7e-4
MnSO4 x 4 H2O 1.0e-5 4.5e-5
Co(NO3)2 5.0e-6 2.7e-5
Na2MoO4 x 2 H2O 5.0e-6 2.1e-5
CuSO4 x 5 H2O 2.5e-8 1.0e-7
CaSO4 - -
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Solutions:
  • 5 ml Trace element solution

156 ANAEROTIGNUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 4 -
L-Alanine 3 33.674
Peptone 3 -
NaHCO3 1 11.904
L-Cysteine HCl x H2O 0.3 1.708
MgSO4 x 7 H2O 0.1 0.406
FeSO4 x 7 H2O 0.018 0.065
Sodium resazurin 5.0e-4 0.002
Potassium phosphate buffer - 5
CaSO4 - -
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Gas phase:
  • 100 % N2

185 THERMOPROTEUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulfur 10 311.818
Starch 5 -
FeSO4 x 7 H2O 0.556 2
Na2S x 9 H2O 0.5 2.082
MgSO4 x 7 H2O 0.492 1.996
CaSO4 x 2 H2O 0.344 1.998
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Solutions:
  • 1 ml Trace elements solution

371 NATRONOBACTERIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 200 3422.31
Agar 20 59.465
Na2CO3 5 47.175
Casamino acids 5 -
Yeast extract 5 -
NH4Cl 1 18.695
KCl 1 13.414
KH2PO4 1 7.348
Na2-glutamate 1 5.913
MgSO4 x 7 H2O 0.24 0.974
CaSO4 x 2 H2O 0.17 0.987
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Solutions:
  • 1 ml Trace element solution SL-10

557 CLOSTRIDIUM SP. MEDIUM (CREATININE)

Compound Conc. [g/L] Conc. [mM]
Creatinine 5.484 48.476
K2HPO4 5.314 30.51
Yeast extract 4.985 -
KH2PO4 2.632 19.342
NaCl 0.997 17.06
NaHCO3 0.997 11.868
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
MgSO4 x 7 H2O 0.499 2.023
CaSO4 x 2 H2O 0.05 0.29
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Solutions:
  • 1 ml Trace element solution SL-11
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2

573 THIOTHRIX MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Na2S x 9 H2O 0.3 1.249
NH4Cl 0.2 3.739
Na-acetate 0.1 1.219
K2HPO4 0.01 0.057
MgSO4 x 7 H2O 0.01 0.041
FeSO4 x 7 H2O 0.004 0.013
EDTA 1.0e-3 0.003
H3BO3 5.0e-5 8.1e-4
ZnSO4 x 7 H2O 5.0e-5 1.7e-4
MnSO4 x 4 H2O 1.0e-5 4.5e-5
Co(NO3)2 5.0e-6 2.7e-5
Na2MoO4 x 2 H2O 5.0e-6 2.1e-5
CuSO4 x 5 H2O 2.5e-8 1.0e-7
CaSO4 - -
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Solutions:
  • 3 ml neutralized Na2S x 9 H2O solution, 10% (w/v)
  • 5 ml Trace element solution

630 MODIFIED THERMUS 162 MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 28 83.252
Na2HPO4 x 12 H2O 4.3 30.29
Tryptone 2.5 35.172
Yeast extract 2.5 -
KH2PO4 0.544 3.998
MgCl2 x 6 H2O 0.2 0.984
CaSO4 x 2 H2O 0.04 0.232
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Solutions:
  • 0.5 ml Trace element solution
  • 100 ml Phosphate buffer

878 THERMUS 162 MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 28 83.252
Na2HPO4 x 12 H2O 4.3 30.29
Tryptone 1 14.069
Yeast extract 1 -
KH2PO4 0.544 3.998
MgCl2 x 6 H2O 0.2 0.984
Nitrilotriacetic acid 0.1 0.523
CaSO4 x 2 H2O 0.04 0.232
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Solutions:
  • 0.5 ml Trace element solution
  • 100 ml Phosphate buffer
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