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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Na2HPO4 x 2 H2O

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81 MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
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Solutions:
  • 5 ml Standard vitamin solution
  • 50 ml Solution A
  • 920 ml Solution B
  • 20 ml Solution C
  • 5 ml Trace element solution SL-6

81a THIOMONAS ISLANDICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 2.868 20.206
K2HPO4 2.275 13.061
NH4Cl 0.989 18.492
NaHCO3 0.495 5.887
MgSO4 x 7 H2O 0.495 2.007
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Solutions:
  • 5 ml Trace element solution SL-6
  • 1 ml Seven vitamins solution
Gas phase:
  • 53 % H2
  • 27 % N2
  • 20 % CO2

86 CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 1 14.069
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
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86a MODIFIED CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 25 74.332
Peptone 3 -
Monosodium glutamate 1 5.913
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
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87 CHLOROFLEXUS MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 0.947 -
Glycyl-glycine 0.947 -
NaNO3 0.473 5.571
NaCl 0.095 1.62
KNO3 0.095 0.937
Na2HPO4 x 2 H2O 0.095 0.667
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Solutions:
  • 1 ml Trace element solution SL-6

87a CHLOROFLEXUS AGGREGANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glycyl-glycine 0.952 -
Yeast extract 0.952 -
NaNO3 0.476 5.603
NaCl 0.095 1.63
KNO3 0.095 0.942
Na2HPO4 x 2 H2O 0.095 0.671
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Solutions:
  • 1 ml Trace element solution SL-6
  • 1 ml Vitamin solution CA

134 HALISCOMENOBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 2 11.101
Glutamic acid 1.31 8.904
MgSO4 x 7 H2O 0.075 0.304
CaCl2 x 2 H2O 0.05 0.34
Na2HPO4 x 2 H2O 0.04 0.282
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Solutions:
  • 1 ml Trace element solution SL-6

260 BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 2 H2O 4.5 31.699
Na-pyruvate 1.5 13.631
KH2PO4 1.5 11.023
NH4Cl 1 18.695
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Solutions:
  • 3 ml Trace element solution SL-6

261 MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 2 H2O 4.5 31.699
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
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Solutions:
  • 3 ml Trace element solution SL-6
Gas phase:
  • 45 % H2
  • 10 % CO2
  • 5 % O2

334 METHANOTHRIX MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-acetate 6.753 82.317
Na2CO3 1.241 11.712
Na2HPO4 x 2 H2O 0.854 6.016
Na2S x 9 H2O 0.497 2.067
NH4Cl 0.298 5.57
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Solutions:
  • 951 ml Solution A
  • 25 ml Solution B
  • 10 ml Solution C
  • 10 ml Solution D
  • 1 ml Solution E
  • 10 ml Solution F
  • 10 ml Trace elements solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2
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