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1004. ANAEROLINEA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.14 g 0.138 1.017
2 0.20 g 0.198 0.972
3 0.15 g 0.148 1.008
4 0.54 g 0.534 9.975
5 Trace element solution SL-11 1.00 ml - -
6 Selenite-tungstate solution 1.00 ml - -
7
(0.1% w/v)
0.50 ml 4.9e-4 0.002
8 2.50 g 2.47 29.407
10 Vitamin solution 10.00 ml - -
12 0.25 g 0.247 1.407
13 0.25 g 0.247 1.029
14 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except bicarbonate, yeast extract, vitamins, glucose and reducing agents), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add solid bicarbonate and adjust pH to 7.0. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration), glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.0, if necessary.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
11 , required for pH adjustment x - -
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 9
Strains with modifications: 8

Gas composition

  • 100 % N2

Light

21 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaHCO3 2.47 29.407
NH4Cl 0.534 9.975
L-Cysteine HCl x H2O 0.247 1.407
Na2S x 9 H2O 0.247 1.029
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Cultivation metadata from 9 strains BacDive

halophily not 2 strains
halophily slightly 1 strain
pH 6 - 8 7 strains
pH 4 - 6 5 strains
pH 8 - 10 3 strains
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