DSMZ 1024 . NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM

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Culture medium recipe

Vol.
×
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 1 g 1 11.765
NaHCO3 1 g 1 11.904
Na2S2O3 x 5 H2O 1 g 1 4.029
Sulfur powder 3 g 3 11.696
Wolin's vitamin solution 10 ml - -
Modified Wolin's mineral solution II 10 ml - -
Synthetic seawater 1000 ml - -
1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution.
2 Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 1 other media (first defined in Medium 997).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 3000 51334.7
K2HPO4 0.14 g 14 80.379
CaCl2 x 2 H2O 0.14 g 14 95.228
NH4Cl 0.25 g 25 467.377
MgSO4 x 7 H2O 3.40 g 340 1379.44
MgCl2 x 6 H2O 4.18 g 418 2056.04
KCl 0.33 g 33 442.656
NiCl2 x 6 H2O 0.50 mg 0.05 0.386
Na2SeO3 x 5 H2O 0.50 mg 0.05 0.19
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 1 -
Trace mineral solution 10.00 ml - -
Solution occurs in 1 other media (first defined in Medium 997).
Compound Amount Unit Conc. [g/L] Conc. [mM]
C6H9NO6 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x 2 H2O 0.500 g 0.5 2.673
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
Solution occurs in 22 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g - -
MgSO4 x 7 H2O 3.000 g - -
MnSO4 x H2O 0.500 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoSO4 x 7 H2O 0.180 g - -
CaCl2 x 2 H2O 0.100 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.010 g - -
AlK(SO4)2 x 12 H2O 0.020 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.010 g - -
NiCl2 x 6 H2O 0.025 g - -
Na2SeO3 x 5 H2O 0.300 mg - -
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 418 2056.04
KCl 33 442.656
NH4Cl 25 467.377
K2HPO4 14 80.379
MgSO4 x 7 H2O 3 12.172
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Cultivation metadata from 4 strains BacDive

pH 4 - 6 2 strains
pH 6 - 8 2 strains
phyla 1 3 strains
oxygen facultative anaerobe 2 strains
temperature thermophilic 3 strains
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