Name: MODIFIED BIEBL AND PFENNIG'S MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1069 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.50	g	0.5	3.674
CaCl₂ x 2 H₂O	0.15	g	0.15	1.02
MgSO₄ x 7 H₂O	2.00	g	2	8.114
NH₄Cl	0.34	g	0.34	6.356
NaCl	20.00	g	20	342.231
Yeast extract	0.40	g	0.4	-
Ferric citrate (0.1% w/v)	5.00	ml	0.005	0.02
Trace element solution SL-7	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.

Trace element solution SL-7 #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Distilled water	1000	ml	-	-
HCl (25%)	1	ml	0.25	6.857
ZnCl₂	70	mg	0.07	0.514
MnCl₂ x 4 H₂O	100	mg	0.1	0.505
H₃BO₃	60	mg	0.06	0.97
CoCl₂ x 6 H₂O	200	mg	0.2	1.54
CuCl₂ x 2 H₂O	20	mg	0.02	0.117
NiCl₂ x 6 H₂O	20	mg	0.02	0.154
Na₂MoO₄ x 2 H₂O	40	mg	0.04	0.165

All strains for this medium:	1
Strains with modifications:	1

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