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193. DESULFOBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 940 ml - -
2 Solution B 1 ml - -
3 Solution C 30 ml - -
4 Solution D x ml - -
5 Solution E 10 ml - -
6 Solution F 10 ml - -
7 , may stimulate growth of some strains
(5% w/v)
20 mg/l 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10%(v/v) inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3.185 22.421
2 0.20 g 0.212 1.56
3 0.30 g 0.318 5.954
4 7.00 g 7.431 127.151
5 1.30 g 1.38 6.788
6 0.50 g 0.531 7.12
7 0.15 g 0.159 1.083
8 Selenite-tungstate solution 1.00 ml - -
9
(0.1% w/v)
0.50 ml 5.3e-4 0.002
10 940.00 ml 996.088 55291.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 DSM 1744 , DSM 10631 and DSM 16697 10 ml - -
2 DSM 2034 10 ml - -
3 DSM 4660 10 ml - -
4 DSM 6637 10 ml - -
5 DSM 8775 10 ml - -
6 DSM 10711 10 ml - -
7 DSM 14956 10 ml - -
8 DSM 15102 10 ml - -
9 DSM 18843 10 ml - -
10 DSM 25524 10 ml - -
Instructions:
  1. Use 10 ml substrates depending on organism (see below)
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 20 83.27
2 10.0 ml 499.103 27704.4
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 2230.95
2 10.0 ml 998.207 55408.8
3 1.0 g/l 1 -
Instructions:
  1. Add after autoclaving 1.00 g/l yeast extract from a sterile anoxic stock solution.

DSM 4660 #

10
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.1 g 10 106.257
2 10.0 ml 998.207 55408.8
3 Seven vitamins solution 1.0 ml - -
Instructions:
  1. Add after autoclaving 1.00 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration.

DSM 6637 #

10
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.58 g 58 998.638
2 10.00 ml 998.207 55408.8
3 3.40 g/l 3.4 33.629
4 5.00 g/l 5 45.436
Instructions:
  1. Supplement medium with 3.40 g/l KNO3 and omit solution F. Add after autoclaving 5.00 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration.

DSM 8775 #

10
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 2271.82
2 10.0 ml 998.207 55408.8
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 2271.82
2 0.5 g 50 -
3 10.0 ml 998.207 55408.8
4 Seven vitamins solution 1.0 ml - -
Instructions:
  1. Add after autoclaving 1.00 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.6 g 160 4993.46
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.5 g 350 1942.76
2 10.0 ml 998.207 55408.8
3 1.0 g/l 1 -
Instructions:
  1. Add after autoclaving 1.00 g/l yeast extract from a sterile anoxic stock solution.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.62 g 62 202.31
2 10.00 ml 998.207 55408.8
3 Seven vitamins solution 1.00 ml - -
Instructions:
  1. Add after autoclaving 1.00 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 33 198.563
2 10.00 ml 998.207 55408.8
3 Seven vitamins solution 1.00 ml - -
Instructions:
  1. Add after autoclaving 1.00 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

DSM 2034 #

10
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 1837.15
2 10.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.6 g 60 416.369
2 10.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 15
Strains with modifications: 15

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 992.156 55072.9
NaCl 7.049 120.607
Na2SO4 3.021 21.267
Na2CO3 1.514 14.281
MgCl2 x 6 H2O 1.309 6.439
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Cultivation metadata from 15 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 15 strains
oxygen anaerobe 14 strains
oxygen facultative anaerobe 1 strain
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