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193b. DESULFOFABA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 930 ml - -
2 Solution B 1 ml - -
3 Solution C 30 ml - -
4 Solution D 10 ml - -
5 Solution E 1 ml - -
6 Solution F 20 ml - -
7 Solution G 10 ml - -
8 , may stimulate growth at the beginning
(5% w/v)
20 mg/l 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B, D, F and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere.
  2. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
  3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10%(v/v) inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.0 g 3.219 22.661
2 0.2 g 0.215 1.577
3 0.3 g 0.322 6.018
4 7.0 g 7.511 128.515
5 0.5 g 0.536 7.196
6 0.6 g 0.644 3.167
7 Selenite-tungstate solution 1.0 ml - -
8
(0.1% w/v)
0.5 ml 5.4e-4 0.002
9 930.0 ml 996.065 55289.9
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 150 1561.52
2 10.00 ml 998.207 55408.8
10 , might need strain adjustment 0.70 g/l - -
11 , might need strain adjustment 0.30 g/l - -
12 , might need strain adjustment 0.15 g/l - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Seven vitamins solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 75 510.152
2 11.5 g 575 2828.29
3 20.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.203 55353.1
MgCl2 x 6 H2O 12.075 59.392
NaCl 6.971 119.28
Na2SO4 2.988 21.033
Na-propionate 1.497 15.584
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Cultivation metadata from 2 strains BacDive

halophily not 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

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193. DESULFOBACTER MEDIUM 87 %
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