MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1098 . BSM-RC MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1098 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 3.620 g 3.62 61.944
K2HPO4 0.260 g 0.26 1.493
NH4Cl 0.075 g 0.075 1.402
Na2SO4 0.050 g 0.05 0.352
NaNO3 0.850 g 0.85 10.001
L-Sodium lactate 2.240 g 2.24 19.989
Yeast extract 0.200 g 0.2 -
Tryptone 0.500 g 0.5 7.034
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Trace vitamins 10.000 ml - -
Distilled water 935.500 ml - -
1 Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, add the following solution (filter-sterilized):
NaHCO3
(8%) 		52.500 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels under a N2 atmosphere and add H2 at 5% volume of the gas phase.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3 g 3 14.756
CaCl2 x 2 H2O 100 mg 0.1 0.68
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 -
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 3.62 61.944
L-Sodium lactate 2.24 19.989
NaNO3 0.85 10.001
Tryptone 0.5 7.034
K2HPO4 0.26 1.493
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain