J1106
1106 .
MARINE METHANOGENIC MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1106 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 22.00 | g | 22 | 376.454 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| KCl | 0.34 | g | 0.34 | 4.561 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.75 | 13.527 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
|
Yeast extract
(BD-Difco) |
0.20 | g | 0.2 | - | |
| Trace mineral solution | 10.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 930.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Solution occurs in 4
other media (first defined in Medium J1084).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.00 | ml | - | - |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 22.01 | 376.625 |
| MgCl2 x 6 H2O | 2.75 | 13.527 |
| NH4Cl | 0.5 | 9.348 |
| KCl | 0.34 | 4.561 |
| Yeast extract | 0.2 | - |
| Show more | ||
