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JCM 335 . JCM MEDIUM No. 335

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J335 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.765 4.39
KH2PO4 0.75 g 0.735 5.403
Na3-EDTA 0.04 g 0.039 -
FeSO4 x H2O 0.01 g 0.01 0.058
MgSO4 x H2O 0.25 g 0.245 2.036
CaCl2 x H2O 0.03 g 0.029 0.265
NaCl 0.20 g 0.196 3.355
NH4Cl 27.00 mg 0.026 0.495
Na2SO4 2.80 g 2.745 -
Sodium acetate 0.16 g 0.157 1.912
Trace vitamins 10.00 ml - -
Trace element solution 10.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.245 -
Distilled water 1000.00 ml - -
1 Adjust pH to 5.5. Fill the gas phase with H2-CO2 (80:20).

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium J243).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
NaCl 1.000 g 1 17.112
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 0.026 g 0.026 0.099
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
2 Comment: For strain JCM 10134, incubate with a 10-20% inoculum.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.745 -
K2HPO4 0.765 4.39
KH2PO4 0.735 5.403
MgSO4 x H2O 0.245 2.036
L-Cysteine HCl x H2O 0.245 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain