J967
967 .
KD-1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J967 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Bacto peptone
(BD-Difco) |
10.0 | g | 9.804 | - | |
| Urea | 2.0 | g | 1.961 | 32.649 | |
| K2HPO4 | 2.5 | g | 2.451 | 14.072 | |
| KH2PO4 | 3.0 | g | 2.941 | 21.613 | |
| NaCl | 3.0 | g | 2.941 | 50.328 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.196 | 0.796 | |
| Trace vitamins | 10.0 | ml | - | - | |
| Trace element solution | 10.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 8.0. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium J741).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 10.00 | mg | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 20.00 | mg | 0.02 | - | |
| H3BO3 | 10.00 | mg | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 10.00 | mg | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 25.00 | mg | 0.025 | 0.193 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals. | |||||
| 2 Adjust final pH of the medium to 7.0 with KOH. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 8.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Bacto peptone | 9.804 | - |
| NaCl | 2.951 | 50.496 |
| KH2PO4 | 2.941 | 21.613 |
| K2HPO4 | 2.451 | 14.072 |
| Urea | 1.961 | 32.649 |
| Show more | ||
