Neutralized sulfide solution 3% (w/v)
Internal Solution-ID: S34
This solution is used in 19 media and was first defined in medium 28.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2S x 9 H2O | 3 | g | 30 | 124.905 | |
| Distilled water | 100 | ml | - | - | |
| 1 The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. | |||||
Media with this solution
| 28 | PFENNIG'S MEDIUM I |
| 28a | THIORHODOCOCCUS MEDIUM |
| 28b | THIORHODOCOCCUS MEDIUM II |
| 29 | PFENNIG'S MEDIUM II |
| 39 | PFENNIG'S MEDIUM I WITH YEAST EXTRACT |
| 43 | PFENNIG'S MEDIUM I WITH SALT |
| 46 | PFENNIG'S MEDIUM I WITH SALT |
| 87 | CHLOROFLEXUS MEDIUM (modified) |
| 377a | PALAEOCOCCUS MEDIUM |
| 377 | PYROCOCCUS MEDIUM |
| 421 | CHROMATIUM SALEXIGENS MEDIUM |
| 422 | AMOEBOBACTER MEDIUM |
| 507 | CARBOXYDOTHERMUS MEDIUM |
| 829a | DESULFUROBACTERIUM ATLANTICUM MEDIUM |
| 867 | RHODOBACTER VELDKAMPII MEDIUM |
| 901 | ACIDOLOBUS ACETICUS MEDIUM |
| 901a | THERMODESULFOBIUM ACIDIPHILUM MEDIUM |
| 1100 | DESULFACINUM MEDIUM |
| 1761 | PFENNIG'S MEDIUM III |