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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: (NH4)2Ni(SO4)2

Your search received 60 results.

498 PSEUDOTHERMOTOGA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Starch 4.883 -
NaCl 3.379 57.818
MgSO4 x 7 H2O 0.859 3.487
Na2-EDTA 0.752 2.237
MgCl2 x 6 H2O 0.674 3.314
KH2PO4 0.488 3.588
Na2S x 9 H2O 0.488 2.033
Yeast extract 0.488 -
CaCl2 x 2 H2O 0.137 0.93
KCl 0.078 1.048
Nitrilotriacetic acid 0.022 0.115
NaBr 0.012 0.119
MnSO4 x H2O 0.007 0.043
H3BO3 0.004 0.063
(NH4)2Ni(SO4)2 0.003 0.007
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Solutions:
  • 15 ml Modified Wolin's mineral solution
Gas phase:
  • 100 % N2

511 METHANOPYRUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.469 21.981
NaHCO3 0.993 11.821
Na2SO4 0.804 5.663
Na2S x 9 H2O 0.497 2.067
KCl 0.298 3.996
NH4Cl 0.248 4.641
KH2PO4 0.089 0.657
K2HPO4 x 3 H2O 0.07 0.399
NaBr 0.04 0.386
MgSO4 x 7 H2O 0.03 0.121
SrCl2 x 6 H2O 0.018 0.067
Nitrilotriacetic acid 0.015 0.078
H3BO3 0.013 0.209
NaCl 0.01 0.17
MnSO4 x H2O 0.005 0.029
Na2WO4 x 2 H2O 0.002 0.006
(NH4)2Ni(SO4)2 0.002 0.005
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 10 ml Marine trace elements solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

534 MODIFIED SME MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 5.5 27.053
NaHCO3 2 23.808
Na2S2O3 x 5 H2O 2 8.058
KCl 0.65 8.719
NH4Cl 0.15 2.804
K2HPO4 0.15 0.861
NaBr 0.1 0.972
MgSO4 x 7 H2O 0.03 0.122
(NH4)2Ni(SO4)2 0.02 0.051
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Solutions:
  • 10 ml Trace element solution
  • 30 ml Solution A
  • 30 ml Solution B
  • 1000 ml Modified Wolin's mineral solution II
Gas phase:
  • 80 % H2
  • 20 % CO2
  • 0.5 % O2

766 THERMOCOCCUS CHITINOPHAGUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 11.383 194.78
MgCl2 x 6 H2O 5.16 25.383
Na2SO4 1.901 13.385
CaCl2 0.534 4.807
(NH4)2SO4 0.5 3.784
KH2PO4 0.5 3.674
Na2S x 9 H2O 0.5 2.082
KCl 0.322 4.32
HCl 0.296 8.118
NaHCO3 0.093 1.108
Chitin 0.067 -
KBr 0.047 0.391
MgSO4 x 7 H2O 0.045 0.183
Nitrilotriacetic acid 0.023 0.118
H3BO3 0.013 0.204
SrCl2 0.012 0.073
MnSO4 x H2O 0.008 0.044
CoSO4 x 7 H2O 0.003 0.017
ZnSO4 x 7 H2O 0.003 0.009
CaCl2 x 2 H2O 0.002 0.01
FeSO4 x 7 H2O 0.002 0.005
NaF 0.001 0.035
Resazurin 1.0e-3 0.004
NiCl2 x 6 H2O 4.5e-4 0.003
(NH4)2Ni(SO4)2 3.0e-4 7.6e-4
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Solutions:
  • 4 ml Chitin stock solution
  • 15 ml Modified Wolin's mineral solution II
  • 485 ml Artifical sea water

792 PYROLOBUS FUMARII MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.723 13.393
KNO3 0.99 9.793
KH2PO4 0.495 3.638
KCl 0.327 4.383
Na2S x 9 H2O 0.149 0.618
NaBr 0.05 0.481
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.169
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
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Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 1 ml Solution C
  • 10 ml Solution D
  • 1 ml Wolfe's mineral elixir
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % H2
  • 20 % CO2

792a THERMOVIBRIO AMMONIFICANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.725 13.406
KNO3 0.991 9.803
KH2PO4 0.496 3.641
KCl 0.327 4.387
Na2S x 9 H2O 0.149 0.619
NaBr 0.05 0.482
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.17
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
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Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 1 ml Wolfe's mineral elixir
Gas phase:
  • 80 % H2
  • 20 % CO2

792b CAMINIBACTER MEDIATLANTICUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.725 13.406
KNO3 0.991 9.803
KH2PO4 0.496 3.641
KCl 0.327 4.387
Na2S x 9 H2O 0.149 0.619
NaBr 0.05 0.482
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.17
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
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Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 1 ml Wolfe's mineral elixir
Gas phase:
  • 80 % H2
  • 20 % CO2

792c NAUTILIA NITRATIREDUCENS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.739 13.473
KNO3 0.996 9.851
Na2CO3 0.996 9.397
KH2PO4 0.498 3.66
Na2S x 9 H2O 0.448 1.866
KCl 0.329 4.409
NaBr 0.05 0.484
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.17
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
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Solutions:
  • 958 ml Solution A
  • 10 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 15 ml Solution E
  • 1 ml Wolfe's mineral elixir
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

796 ARCHAEOGLOBUS VENEFICUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 7.129 35.064
NaHCO3 3.988 47.473
Na-acetate 0.997 12.154
Na2SO3 0.997 7.91
Na2S x 9 H2O 0.499 2.076
KCl 0.329 4.413
NH4Cl 0.249 4.66
K2HPO4 x 3 H2O 0.179 1.03
NaCl 0.01 0.171
MnCl2 x 4 H2O 0.006 0.029
(NH4)2Ni(SO4)2 0.002 0.005
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Solutions:
  • 1 ml Trace element solution
Gas phase:
  • 40 % H2
  • 40 % N2
  • 20 % CO2

1028 THERMINCOLA MEDIUM (CO)

Compound Conc. [g/L] Conc. [mM]
NH4Cl 0.997 18.639
Na2S x 9 H2O 0.997 4.151
NaHCO3 0.499 5.934
Na2CO3 0.499 4.703
KH2PO4 0.499 3.663
KCl 0.329 4.413
MgCl2 x 6 H2O 0.329 1.618
Na-acetate 0.199 2.431
Yeast extract 0.199 -
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
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Solutions:
  • 1 ml Wolfe's mineral elixir
  • 2 ml Wolin's vitamin solution (10x)
Gas phase:
  • 90 % CO
  • 10 % N2
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