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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Na-silicate

Your search received 13 results.

511 METHANOPYRUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.469 21.981
NaHCO3 0.993 11.821
Na2SO4 0.804 5.663
Na2S x 9 H2O 0.497 2.067
KCl 0.298 3.996
NH4Cl 0.248 4.641
KH2PO4 0.089 0.657
K2HPO4 x 3 H2O 0.07 0.399
NaBr 0.04 0.386
MgSO4 x 7 H2O 0.03 0.121
SrCl2 x 6 H2O 0.018 0.067
Nitrilotriacetic acid 0.015 0.078
H3BO3 0.013 0.209
NaCl 0.01 0.17
MnSO4 x H2O 0.005 0.029
Na2WO4 x 2 H2O 0.002 0.006
(NH4)2Ni(SO4)2 0.002 0.005
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
Na-silicate 9.9e-4 0.008
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 10 ml Marine trace elements solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

514 BACTO MARINE BROTH (DIFCO 2216)

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
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514a HALF STRENGTH BACTO MARINE BROTH

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
NaCl 9.725 166.41
MgCl2 2.95 30.984
Bacto peptone 2.5 -
Na2SO4 1.62 11.405
CaCl2 0.9 8.11
Yeast extract 0.5 -
KCl 0.275 3.689
NaHCO3 0.08 0.952
Fe(III) citrate 0.05 0.204
KBr 0.04 0.336
SrCl2 0.017 0.107
H3BO3 0.011 0.178
Na2HPO4 0.004 0.028
Na-silicate 0.002 0.016
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514b MEDIUM 514 plus additional salt

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
Agar 17.5 52.032
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
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514c REACTIVATION WITH LIQUID MEDIUM 514

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
Agar 18 53.519
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
Na2CO3 0.16 1.51
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
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Solutions:
  • 1000 ml 514. BACTO MARINE BROTH (DIFCO 2216)

514e MARINE BROTH (ANAEROB)

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
MgCl2 5.9 61.968
Peptone 5 -
Na2SO4 3.24 22.811
Yeast extract 1 -
KCl 0.55 7.378
L-Cysteine HCl x H2O 0.3 1.708
Na2S x 9 H2O 0.3 1.249
NaHCO3 0.16 1.905
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
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Gas phase:
  • 100 % N2

514f MARINE BROTH (ROTH CP73)

Compound Conc. [g/L] Conc. [mM]
NaCl 19.4 331.964
Agar 15 44.599
MgCl2 8.8 92.427
Peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
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514g HALF STRENGTH BACTO MARINE BROTH with 50% Seawater

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
NaCl 9.725 166.41
MgCl2 2.95 30.984
Bacto peptone 2.5 -
Na2SO4 1.62 11.405
CaCl2 0.9 8.11
Yeast extract 0.5 -
KCl 0.275 3.689
NaHCO3 0.08 0.952
Fe(III) citrate 0.05 0.204
KBr 0.04 0.336
SrCl2 0.017 0.107
H3BO3 0.011 0.178
Na2HPO4 0.004 0.028
Na-silicate 0.002 0.016
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611 PYROBACULUM (BS) MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.263 11.129
NaHCO3 2 23.808
KNO3 1 9.891
Yeast extract 0.5 -
Na2SO4 0.405 2.851
NH4Cl 0.25 4.674
KCl 0.15 2.012
KH2PO4 0.07 0.514
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaBr 0.013 0.121
Na2CO3 0.013 0.118
NaCl 0.01 0.171
KBr 0.01 0.084
SrCl2 x 6 H2O 0.009 0.034
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
Na2HPO4 0.001 0.007
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 -
NaF 3.0e-4 0.007
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
Fe(NH4)2(SO4)2 x 6 H2O 2.0e-4 -
H3BO3 1.0e-4 0.002
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Na-silicate 5.0e-5 4.1e-4
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Solutions:
  • 125 ml Synthetic seawater mix
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % H2
  • 20 % CO2

760 MARINE THERMOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.45 332.82
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.811
CaCl2 1.8 16.219
Yeast extract 1 -
KCl 0.55 7.378
NaHCO3 0.16 1.905
Fe(III) citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
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Gas phase:
  • 80 % N2
  • 20 % CO2
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