DSMZ 1630b . NITROSOPUMILUS UREIPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 26.00 g 26 444.901
MgSO4 x 7 H2O 5.00 g 5 20.286
MgCl2 x 6 H2O 5.00 g 5 24.594
CaCl2 x 2 H2O 1.50 g 1.5 10.203
KBr 0.10 g 0.1 0.84
KH2PO4
(0.10% w/v)
2.00 ml 0.002 0.015
NaHCO3 0.25 g 0.25 2.976
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NH4Cl 0.05 g 0.05 0.935
Na-pyruvate 0.05 g 0.05 0.454
Wolin's vitamin solution 10.00 ml - -
Catalase
(SIGMA C1345) (0.10% w/v)
2.50 ml 0.003 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, trace elements, selenite-tungstate, ammonium chloride, pyruvate, vitamins, and catalase) and dispense medium in Erlenmeyer flasks with screw caps (e.g., from SCHOTT), then autoclave. Add bicarbonate, trace elements, selenite-tungstate, ammonium chloride, pyruvate and vitamins from stock solutions sterilized by filtration. Adjust pH of the complete medium to 6.8 using a sterile solution of sodium bicarbonate (10% w/v) or 1 N HCl.
2 After inoculation, add to the medium 5 - 10 units/ml catalase from a freshly prepared 1.00 mg/ml stock solution sterilized by filtration.
3 Note: Use at least 5 - 10% (v/v) inoculum and incubate in the dark without shaking.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 14.04.22
Source: DSMZ
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26 444.901
MgCl2 x 6 H2O 5 24.594
MgSO4 x 7 H2O 5 20.286
CaCl2 x 2 H2O 1.5 10.203
NaHCO3 0.25 2.976
Show more