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1630a. NITROSOPUMILUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 26.00 g 25.415 434.879
2 5.00 g 4.888 19.83
3 5.00 g 4.888 24.041
4 1.50 g 1.466 9.974
5 0.10 g 0.098 0.821
6
(0.1% w/v)
2.00 ml 0.002 0.014
7 HEPES buffer solution 10.00 ml - -
8 0.05 g 0.049 0.914
9 Trace element solution SL-11 1.00 ml - -
10 0.05 g 0.049 0.444
11 0.25 g 0.244 2.909
12 Vitamin solution 10.00 ml - -
13 1000.00 ml 975.764 54163.1
Instructions:
  1. Dissolve ingredients (except trace elements, HEPES buffer, ammonium chloride, pyruvate, bicarbonate and vitamins), dispense medium into Erlenmeyr flasks with screw caps (e.g., 20 ml medium per 100 ml flask) and autoclave. Add HEPES buffer and ammonium chloride from stock solutions sterilized by autoclaving. Trace elements, pyruvate, bicarbonate and vitamins are added from stock solutions sterilized by filtration. Adjust pH of the complete medium to 7.2 using sterile solutions of bicarbonate (5% w/v) or 1 N HCl.
  2. Note: Use at least 5%(v/v) inoculum and incubate in the dark without shaking.
Solution occurs in 1 other media (first defined in medium 1630 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(pellets)
24.0 g 24 600.044
2
(free acid)
238.4 g 238.4 -
3 1000.0 ml 998.207 55408.8
Instructions:
  1. Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.255 55300.5
NaCl 25.415 434.879
MgCl2 x 6 H2O 4.888 24.041
MgSO4 x 7 H2O 4.888 19.83
HEPES 2.33 -
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