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1630. NITROSOSPHAERA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.979 16.759
2 0.40 g 0.392 1.927
3 0.10 g 0.098 0.666
4 0.20 g 0.196 -
5 0.50 g 0.49 6.569
6 0.05 g 0.049 0.345
7 HEPES buffer solution 10.00 ml - -
8 0.05 g 0.049 0.916
9 Trace element solution SL-11 1.00 ml - -
10 0.05 g 0.049 0.445
11 0.25 g 0.245 2.915
12 Vitamin solution 10.00 ml - -
13 1000.00 ml 977.676 54269.2
Instructions:
  1. Dissolve ingredients (except trace elements, HEPES buffer, ammonium chloride, pyruvate, bicarbonate and vitamins) and dispense medium under air atmosphere into culture vessels suitable for anaerobic cultivation (e.g., 20 ml medium per 100 ml serum bottle), seal with grey butyl rubber stoppers and autoclave. Add HEPES buffer and ammonium chloride from stock solutions sterilized by autoclaving. Trace elements, pyruvate, bicarbonate, and vitamins are added from sterile stock solutions sterilized by filtration. Adjust pH of the complete medium to 7.5 using a sterile solution of bicarbonate (5% w/v) or 1 N HCl.
Solution occurs in 1 other media (first defined in medium 1630 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(pellets)
24.0 g 24 600.044
2
(free acid)
238.4 g 238.4 -
3 1000.0 ml 998.207 55408.8
Instructions:
  1. Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
HEPES 2.335 -
NaCl 0.979 16.759
KCl 0.49 6.569
MgCl2 x 6 H2O 0.392 1.927
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