MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1630e . Synthetic Crenarchaeota Medium (SCM)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Basic salt water medium #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 26.0 g 26 444.901
MgSO4 x 7 H2O 5.0 g 5 20.286
MgCl2 x 6 H2O 5.0 g 5 24.594
CaCl2 x 2 H2O 1.5 g 1.5 10.203
KBr 0.1 g 0.1 0.84
NaHCO3 buffer (1M) 2.0 ml - -
HEPES buffer solution 10.0 ml - -
KH2PO4 solution (2.93 nM) 5.0 ml - -
Trace element solution (Nitrososphaera) 1.0 ml - -
FeNaEDTA solution (7.5 mM) 1.0 ml - -
NH4Cl (1 M) 2.0 ml - -
Double distilled water 1000.0 ml - -
1 Incubate all used glass ware and magnetic stirring rods in 0.1 M HCl and rinse 3x with double distilled water to remove residual detergents (always check visual for soap bubbles).
2 Autoclave the basic salt water medium and cool to room temperature. This medium should be free of any precipitations!
3 Aseptically, add per L of basic salt water medium the following solutions from sterile stocks (do not store complemented SCM): Trace element solution (Nitrososphaera), FeNaEDTA solution, NaHCO3 solution, HEPES buffer solution, NH4Cl solution, KH2PO4 solution
4 Adjust pH to 7.6.

HEPES buffer solution #

1000
Solution occurs in 3 other media (first defined in Medium 1630).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH
(pellets) 		24.0 g 24 600.045
HEPES
(free acid) 		238.4 g 238.4 1000.42
Distilled water 1000.0 ml - -
1 Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.

Trace element solution (Nitrososphaera) #

1000
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(12.5M) 		8 mL 3.646 100
H3BO3 30 mg 0.03 0.485
MnCl2 x 4 H2O 100 mg 0.1 0.505
CoCl2 x 6 H2O 190 mg 0.19 1.463
NiCl2 x 6 H2O 24 mg 0.024 0.185
CuCl2 x 2 H2O 2 mg 0.002 0.012
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Double distilled water 1000 ml - -
1 This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.

FeNaEDTA solution (7.5 mM) #

1000
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 2753 mg 2.753 -
Double distilled water 1000 ml - -
1 Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.

NaHCO3 buffer (1M) #

50
Solution occurs in 2 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4.2 g 84 999.917
Double distilled water 50.0 ml - -
1 Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 µm) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 °C. Avoid high headspace to liquid ratios to ensure stable concentration.

NH4Cl (1 M) #

50
Solution occurs in 3 other media (first defined in Medium 1630c).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 2.67 g 53.4 998.317
Double distilled water 50.00 ml - -
1 Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
2 Filtrate (0.2 µm).

KH2PO4 solution (2.93 nM) #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.4 g 0.4 2.939
Double distilled water 1000.0 ml - -

alpha-ketoglutamate solution (20mM) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
alpha-ketoglutamate 20 mM - 20
Double distilled water 1000 ml - -

Metadata

Last modified: 17.11.23
Source: MediaDive: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.6
Equipment needed: Autoclave, Filter
Reference: doi.org/10.1038/nature03911
Edit in Medium builder

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26 444.901
MgCl2 x 6 H2O 5 24.594
MgSO4 x 7 H2O 5 20.286
HEPES 2.384 10.004
CaCl2 x 2 H2O 1.5 10.203
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain