1
1 .
NUTRIENT AGAR
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Rhodococcus sp. DSM 6427
* Modification: Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 1 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , for solid medium | 15 | g | 15 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Metadata
| Last modified: | 20.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Taxonomic range: | Phages |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | - |
| Peptone | 5 | - |
| Meat extract | 3 | - |
| Distilled water | - | - |
Cultivation metadata from 2324 strains
| halophily | non-halophilic | 7 strains |
| halophily | halotolerant | 1 strain |
| pH | 6 - 8 | 150 strains |
| pH | 4 - 6 | 95 strains |
| pH | 8 - 10 | 85 strains |
| Show more | ||