Culture medium recipe
Vol.
×
Main sol. 118 #
ml
| # | Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|---|
| 2 | Main sol. 1 | 1000 | ml | - | - |
| 3 | DL-diaminopimelic acid | 100 | mg | 0.1 | - |
Instructions:
- To medium 1 add 100 mg DL-diaminopimelic acid.
| # | Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|---|
| 1 | Peptone | 5 | g | 5 | - |
| 2 | Meat extract | 3 | g | 3 | - |
| 3 |
Agar
optional |
15 | g | 15 | - |
| 4 | Distilled water | 1000 | ml | 998.207 | 55408.8 |
| 5 |
MnSO4 x H2O
, recommended for sporulation of Bacillus strains
optional |
10 | mg | 0.01 | 0.059 |
Instructions:
- Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
No comments for this medium.