Culture medium recipe
Vol.
×
Main sol. 118 #
ml
Equipment needed: Autoclave
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 5 | g | 5 | - | |
Meat extract | 3 | g | 3 | - | |
Diaminopimelic acid | 100 | mg | 0.1 | 0.526 | |
Agar , if necessary | 15 | g | 15 | 44.599 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. |
No comments for this medium.