429c
429c .
COLUMBIA-GLUCOSE-CYSTEINE-AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Allofrancisella guangzhouensis DSM 102975
* Modification: Plus 5% CO2
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 429c #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia agar base | 1000.0 | ml | - | - | |
| Horse blood OR Sheep blood | 4.0 | % | 40 | - | |
| Glucose | 1.0 | % | 10 | 55.506 | |
| Cysteine | 0.1 | % | 1 | 8.254 | |
| 1 Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50°C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. | |||||
Solution occurs in 22
other media (first defined in Medium 220).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 15 | g | - | - | |
| Soy peptone | 5 | g | - | - | |
| NaCl | 5 | g | - | - | |
| Agar | 15 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||
Solution occurs in 5
other media (first defined in Medium 215).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Brain heart infusion
(Difco) |
37 | g | 37 | - |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 19.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 5 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Horse blood | 40 | - |
| Sheep blood | 40 | - |
| Glucose | 10 | 55.506 |
| Cysteine | 1 | 8.254 |
| Columbia agar base | - | - |
